allantoic fluid meaning in Chinese
尿囊液
Examples
- Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna试剂盒提取病毒rna后，逆助兴才李医‘笋脂硕圣‘老‘才转录合成cdna 。 - Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna提取试剂盒按说明提取，提取的rna逆转录合成cdna ， cdna为pgr反应扩增的模板，特异性的引物是针对各个基因片段设计的。 - Two ndv strains , lasota and f48e9 , were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37 , then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，将ndvlasota株和f48e9株分别接种于9日龄spf鸡胚，于37孵化36h ，置4 12h后收获鸡胚尿囊液。 - Heamagglutination tests were applied to detect virus in allantoic fluid of chicken embryos which were infected by b95 gathered from the vaccinated chickens " cloacal and oral cavity . the results show that the virus may be detected from 2 days to 11 days after the chicken being vaccinated . the hi antibodies were measured by heamagglutination inhibition tests . there is no significant difference between the immunized and the control chickens which were fed in one case . chickens were immunized with b95 by different immunization meathods or with different vaccines by the same meathod . lt is demonstrated that eyedrop , drinking water , spray or muscle injection all can stimulate good effects , but eyedrop and spray seem to be the best meathods . b95 immunized chicken have relatively higher hi titers and it also can last for a longer time than others
但如果两者相隔10天以上免疫， b95免疫不受h120的影响；如果同时免疫b95和h120 ，加大b95的免疫剂量也能获得良好的免疫效果。用棉拭子采b95免疫鸡口腔、泄殖腔的分泌液，检测其中病毒的存在，结果免疫后2 11天鸡口腔和泄殖腔中均有病毒的存在，说明b95免疫鸡带毒时间长。研究结果表明， b95具有不受母源抗体干扰、 hi抗体产生快、水平高、持续时间长、同居扩散性强等特点，因此b95是一株优良的、具开发前景的新的新城疫疫苗株。 - Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。